This is a strengthen the argument question - we're asked to find an answer choice that helps support the conclusion and/or the reasoning that leads the author to make such a conclusion. Let's analyze the stim first. The core of this argument is:
group 3 used new toothbrush + groups 1 and 2 used same toothbrush ---> Group 3 had less plaque buildup at the end of the study than groups 1 and 2
Right off the bat you should be thinking, "why were the results different between group 3 and 1/2?"
Also, you should take note of the fact of the differences between the two groups that participated in the study; ie) the usage of a new and old toothbrush.
Answer choice B) helps explain why group 3 had less plaque buildup than the other two groups. It's because new toothbrushes have bristles that are stiff enough to inhibit the buildup of plaque.
A) is wrong because it only explains why group 1 might have had the plaque buildup - they didn't sterilize their brush every month. But this A/C leaves open the possibility that group 2 (who DID sterilize every month) could also have less plaque buildup. And of course, the conclusion says they had more than group 3.
C) Method is irrelevant
D) Only once a day? this might weaken the argument if anything
E) How vigorously they brushed is irrelevant, also it this A/C claims they brushed equally vigorously, so if anything, that should lead to equal amounts of plaque among the three groups.